Journal: Scientific Reports
Article Title: A microfluidics platform for simultaneous evaluation of sensitivity and side effects of anti-cancer drugs using a three-dimensional culture method
doi: 10.1038/s41598-024-84297-0
Figure Lengend Snippet: Establishment of a microfluidic system using an organ-on-chip system and feline mammary tumor (FMT) organoids. Overview of organoids-on-a-chip experiments ( A ). Representative H&E-stained image (Left panel) and expression of a mammary tumor marker HER2 (Right panel) in FMT organoids are shown ( B ). Scale bar: 50 μm. After generating organoids from a cat with mammary tumor disease, the organoids were mounted on a microfluidic device, and drug perfusion was performed. After perfusion, the organoids were collected for genetic analysis and cell survival measurements. The device was created by CAD design and acrylic cutting. The width and height of the flow channel were 0.4 and 0.5 mm, respectively, and the diameter of the chamber was set at 5 mm ( C ). Liquid flows in from the left side of the device and flows out in three separate directions ( D ). The device was connected to a syringe pump and perfused in a CO 2 incubator at 37 ℃ for 48 h ( E ).
Article Snippet: After milling the microfluidic features, a 1 mm diameter drill bit (MHR230, NS Tool Co., Ltd., Japan) was used to segment the acrylic sheet into individual sections, each measuring 3 × 4 cm and containing a complete microfluidic channel network.
Techniques: Staining, Expressing, Marker